Abstract
Bone marrow (BM) derived dendritic cells (DC) are the most potent stimulator cells of primary mixed leukocyte reactions (MLR) and also serve as powerful antigen-presenting cells in the priming of CD4+ T cells in vivo. The investigation of mechanisms by which DC function, both in vivo and in vitro, requires optimal conditions for culture of these cells so that large numbers can be produced for analysis. With the availability of recombinant (r) GM-CSF, several reports have shown that it is possible to grow many DC from murine1 and human2 BM. Although murine rGM-CSF has been used in the rat to promote the survival of lymph-borne DC for up to 72 hours3, until this report cytokines have not been used to enhance the DC yield from fresh rat BM cultures.KeywordsBone MarrowDendntlc CellAdherent CellRepresentative DataCulture MaterialThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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