Abstract
A manual method is described in which nitrate is reduced to nitrite in 2 h by hydrazine in the presence of copper; the reducing medium is buffered to pH 10 with cyclohexyl-aminopropane sulfonic acid and sodium hydroxide. No special apparatus is required, and reduction is not affected by normal laboratory lighting or changes in ambient temperature within the range of 23 to 27°C. Absorbances are linear for concentrations from 1 to 20 mg NO 3-N/l. The standard deviations for samples containing 5 and 20 mg NO 3-N/l were 0.1 and 0.2, respectively.
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