Abstract
PRESENT methods for the production of Clostridium histolyticum collagenase depend chiefly on growth of the organism in complex culture media1,2. It is the purpose of this communication to describe a relatively simple, partially defined medium for the production of large quantities of highly active fermentation broths of Cl. histolyticum collagenase. The medium described appeared to facilitate the isolation and purification of collagenases which were essentially free of non-specific proteinases3.
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