Abstract
Having been impressed with the importance of the Gram stain as a routine laboratory procedure in bacteriologic examinations, and with the fact that, as ordinarily performed, it is time-consuming (involving from five to ten minutes), and comparatively laborious, I evolved the following simple technic, with the idea of eliminating the time element as far as possible without impairing its reliability: Six wide-mouthed, 4-ounce bottles are numbered from 1 to 6 and labeled according to the following ingredients: Bottle 1 contains alcohol-saturated gentian-violet with distilled water in the proportion of 1 part stain to 3 parts water. Bottle 2 contains plain distilled water, Bottle 3 Gram's solution, Bottle 4 absolute methyl alcohol, Bottle 5 plain distilled water and Bottle 6 dilute Ziehl-Neelsen carbolfuchsin (about 1:10), whose speedy and penetrating staining properties are well known. The elements of the stain differ from the original technic in two essential details, namely, the employment
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