Abstract
The purpose of this study is to determine the properties of the solvents responsible of the chromatographic phenomenon. A simple and homogeneous system of ascending chromatography on paper is described, allowing varying only one of the factors constituting it. This method made it possible to determine constants by means of a simple equation. The validity of the method is deduced from the good agreement between its mathematical expression and the experimental values obtained.
Highlights
The technique named “chromatography” got its credentials early in 1903 by Tswett [1] who’s Russian name means “color”
- C00 is the common endpoint expressed in terms of Rf of a homologous series of salts corresponding to the zero salt concentration of the eluent
Halides of Monovalent Salts The Rf are plotted on the y-axis while the volume dilutions (Equation (1)) as well as their reduction by their molecular weight respective are potted on the x-axis
Summary
The technique named “chromatography” got its credentials early in 1903 by Tswett [1] who’s Russian name means “color”. It has exploded since 1940 and has been the subject of many practical publications in the fields of chemistry, physics and biology. In 1931 Kuhn and Lederer [3] rediscovered the separate power of column chromatography. Improvements and evolutions have led the original chromatography technique to flourish in a wide range of specialized developments [5] [6] with simple applications in the separation of sugar and amino acids [7] [8]. Gas chromatography has brought a rigor that has developed many theories on this
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