Abstract
A technique for rapidly collecting blood of testicular origin is described, one which can provide sufficient plasma amounts to investigate some steps of testicular steroid biogenesis in vivo in 2 species. In adult male rats, testosterone (T), androstenedione (4A) and 5-androstenediol (5AD) were determined in pampiniform plexus testicular venous blood (PPTV) and peripheral (PV) blood samples before and 2 h after human Chorionic Gonadotropin (hCG). PPTV concentration of 5AD was 0.83±0.1 ng/ml (mean ± SEM) with a PPTV/PV ratio of 7.0±1.0, comparable to a PPTV/PV ratio for 4A of 5.8±1.8. After hCG, PPTV concentration of 5AD significantly increased to 1.28±0.15 ng/ml ( P < 0.05). Those data are in favor of a participation of 5-ene pathway to testicular biogenesis of T associated to a 4-ene pathway which is predominant. In adult male Macaca fascicularis, spermatic vein (SV) concentrations of 5AD and 4A were comparable (3.0±1.2 vs 4.3±1.0 ng/ml) as well as SV/PV ratios under basal conditions (3.5±0.9 vs 5.1±0.1), as well as 48 h after hCG, confirming in vivo that both 5-ene and 4-ene pathways are involved in testicular T biogenesis. Testicular production of estradiol (E2), estrone (E1) and their sulfates E2S and E1S showed a SV/PV ratio significantly higherthan 1 (3.4±0.6; 2.4±0.1; 1.7±0.2 and 1.6±0.2, respectively).
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