Abstract
In this work, a simple and enzyme-free surface plasmon resonance (SPR) biosensing strategy has been developed for highly sensitive detection of two major PML/RARα (promyelocytic leukemia, retinoic acid receptor alpha) subtypes based on the heterogeneous fusion gene-triggered nonlinear hybridization chain reaction (HCR). On the gold chip surface, the cascade self-assembly process is triggered after the introduction of PML/RARα. The different fragments of PML/RARα can specifically hybridize with capture probes (Cp) immobilized on the chip and the hybridization DNA1 (H1). Then, the nonlinear HCR is initiated by the complex of Cp-PML/RARα-H1 with the introduction of two hybridization DNA chains (H2 and H3). As a result, a dendritic nanostructure is achieved on the surface of chip, leading to a significant SPR amplification signal owing to its high molecular weight. The developed method shows good specificity and high sensitivity with detection limit of 0.72 pM for “L” subtype and 0.65 pM for “S” subtype. Moreover, this method has been successfully applied for efficient identification of clinical positive and negative PCR samples of the PML/RARα subtype. Thus, this developed biosensing strategy presents a potential platform for analysis of fusion gene and early diagnosis of clinical disease.
Highlights
PML/RARα is the product of the chromosomal translocation t (15; 17) (q22; q21) and occurs in acute promyelocytic leukemia (APL)[1,2]
To simplify the number of DNA chains for nonlinear hybridization chain reaction (HCR) reaction and facilitate the protocol of detection, a novel surface plasmon resonance biosensing strategy has been developed for detection of two major PML/RARα subtypes based on heterogeneous fusion gene-triggered nonlinear HCR
On the gold chip surface, the cascade self-assembly process is triggered after the introduction of PML/ RARα used as linker
Summary
Received: 17 May 2017 Accepted: 10 October 2017 Published: xx xx xxxx resonance biosensor for detection of PML/RARα based on heterogeneous fusion genetriggered nonlinear hybridization chain reaction. A simple and enzyme-free surface plasmon resonance (SPR) biosensing strategy has been developed for highly sensitive detection of two major PML/RARα (promyelocytic leukemia, retinoic acid receptor alpha) subtypes based on the heterogeneous fusion gene-triggered nonlinear hybridization chain reaction (HCR). This method has been successfully applied for efficient identification of clinical positive and negative PCR samples of the PML/RARα subtype This developed biosensing strategy presents a potential platform for analysis of fusion gene and early diagnosis of clinical disease. The strategy has been successfully applied for identification of clinical positive and negative PCR samples of the PML/RARα subtype This developed SPR biosensing strategy has great potential application for detection of fusion gene and early diagnosis of clinical disease
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