Abstract
Statistical methods for the analysis of initial-velocity and/or inhibition data are described. They involve application of F tests (i) to determine goodness of fit to the first-order Michaelis-Menten equation, (ii) to predict the reaction mechanism by assessing slope and y-intercept effects in Lineweaver-Burk plots according to the inspection rules of Cleland [Cleland, W. W. (1963) Biochim. Biophys. Acta 67, 188–196], (iii) to test the linearity of the replots of slopes or y-intercepts versus the reciprocal of the substrate concentration or the inhibitor concentration, and (iv) to estimate the true K m or K i values from these replots. The method serves to fill a gap in the kinetic analysis methodology between the antiquated graphical method and the sophisticated direct computer-fitting of data to a variety of possible rate equations. The entire theoretical and computational format is provided to allow the investigator to apply these statistical tests to his data using only a desk-top calculator.
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