Abstract
A staining technique was developed for locating the sporozoites of Eimeria spp. in both mammalian and avian hosts. Bouin-fixed tissues embedded in Paraffin wax and cut at a thickness of 3 micrometers were stained by a controlled sequence of Ehrlich's haematoxylin, acid fuchsin and aniline blue-orange G-acetic acid which took only 30 min to perform. The refractible globule of the sporozoite stained a brilliant crimson whilst the tissue had an overall blue colour, thus enabling rapid scanning of material under low-power magnification. The granules of eosinophils and heterophils also stained a brilliant crimson and their significance in immunological responses indicates a wider application for this technique.
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