Abstract
Cloud point extraction (CPE) method was successfully used for estimation and preconcentration of Allura red (E129) by spectrophotometry. The procedure was depended on the extraction of Allura red as an ion pair with cetyl pyridinium chloride from aqueous solution using a nonionic surfactant TX-114 or Brij-35. The absorbance of the extracted surfactant- rich phase was measured at 507 nm after diluted with water.The influence of different factors on cloud point extraction(CPE) of Allura red such as pH media ,equilibrium temperature ,incubation time , concentration of surfactant and electrolyte concentration was investigated.Calibration graph was linear in a concentration range between 2-40 µgml-1 of Allura red with correlation coefficient r= 0.9998 for TX-114 and 0.9992 for Brij-35 .The developed procedure was applied to estimation and preconcentration of Allura red in the different beverages
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