Abstract
Generally, the measurement of three-dimensional (3D) swimming behavior in zebrafish relies on commercial software or requires sophisticated scripts, and depends on more than two cameras to capture the video. Here, we establish a simple and economic apparatus to detect 3D locomotion in zebrafish, which involves a single camera capture system that records zebrafish movement in a specially designed water tank with a mirror tilted at 45 degrees. The recorded videos are analyzed using idTracker, while spatial positions are calibrated by ImageJ software and 3D trajectories are plotted by Origin 9.1 software. This easy setting allowed scientists to track 3D swimming behavior of multiple zebrafish with low cost and precise spatial position, showing great potential for fish behavioral research in the future.
Highlights
Zebrafish has emerged as a promising new organism for research on anxiety due to their robust cortisol stress response, behavioral strain differences, and sensitivity to drug treatments or predators as well as their change in alarm pheromones [9,10,11,12]
We found that the average speeds and rapid movement time ratio of zebrafish were significantly increased in the 2% EtOH-treated group (Figure 3A,B)
Whilst 3D movement tracking could be done with a higher number of zebrafish (10 fish) in the current novel tank, the percentage of identities reliability was low
Summary
A higher frame rate gives a more accurate tracking result, takes a longer time to video to reduce gap border distraction in the borders of tank Non-transparent shields on both the left and right sides to prevent distraction affecting the zebrafish light platform (by ZGene Biotech Inc., http://www.zgenebio.com/) as a background light source to during recording. The fish must be adapted to the new such as (1) the gender of the fish (in certain temperatures, male fish swim faster than female fish [41]), environment for at least 15 min before capturing the video in order to reduce anxiety and increase
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