Abstract
Inositol (1,4,5)P 3 (IP 3) receptors in rat cerebellar membranes have been used to develop a radioreceptor assay for endogenous IP 3. The assay is sensitive, detecting as little as 1 pmole IP 3 in a 0.5 ml volume. The receptor is highly selective for the 1,4,5-isomer of IP 3 so that assays can employ crude tissue extracts with no purification. Chromatographic analysis and selective enzymatic hydrolysis of IP 3 show that in crude tissue extracts only authentic IP 3 is detected. A hundred or more samples can be assayed in a day for endogenous IP 3.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
