Abstract
Abstract A rapid, sensitive, inexpensive and specific method for identification and quantitation of methotrexate present in pharmaceutical dosage forms or in bulk drug is described. Methotrexate is completely separated from its disintegration products by reversed phase thin layer chromatography on silanized silicagel HF 254. The solvent system being citrate phosphate buffer pH 6.0 : methanol :: 70 : 30. The buffer contained 4.62% of disodium hydrogen phosphate and 0.7% citric acid. The plate was observed under short wave UV and methotrexate was assayed cither by dual wave length densitometry at 302 nm with a background correction at 333 nm or by measuring optical density at 302 nm after extraction from the plate with methanol : 0.1 N HCl :: 1 : 1 by conventional method. Superiority of the proposed method over HPLC and paper chromatographic methods is described.
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