Abstract
A simple and rapid method for simultaneous preparation of individual protein fractions from glandless cottonseed storage proteins was developed. With gel filtration chromatography, using Sephacryl S-300 HR and 10% NaCl solution as an eluent, 3 fractions, FI, FII, FIII were obtained. FII and FIII produced single peaks by gel filtration rechromatography and anion exchange chromatography. FII and FIII showed both a single major and a barely noticeable minor band, respectively, on polyacrylamide gel electrophoresis. Analytical ultracentrifuge patterns showed impurites less than 2% in FII and a single symmetrical peak for FIII. Sedimentation coefficients were computed to be 10.7S and 6.8S for FII and FIII, respectively; molecular weights by osmometry were 2.39 × 105 and 1.40 × 105 g/mole for FII and FIII, respectively. Both fractions were high in acidic and low in sulfur-containing amino acids, and contained phytate and gossypol at less than 10 and 2 ppm, respectively.
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