Abstract
A radiometric assay for aryl hydroxylase activity in liver microsomal preparations has been devised. It involves incubation of benzenesulfonanilide-4′- 3H with enzyme followed by separation of substrate and product from tritiated water with a small charcoal column. The release of tritiated water is stoichiometric with formation of 4′-hydroxybenzenesulfonanilide. A similar assay is based on the hydroxylation of acetanilide-4- 3H, but has the disadvantage of variable release of tritium as tritiated water. “Benzenesulfonanilide hydroxylase” activity is carbon monoxide sensitive and appears to be induced by pretreatment of animals with phenobarbital but not by pretreatment with 3-methylcholanthrene.
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