Abstract
The raw material originating from freshly caught Antarctic krill (E. superba) was processed by centrifugation and then immediately frozen. This squeezed fluid adjusted to pH 7 was subjected to spontaneous autolysis by storing at different temperatures (20-45 degrees C) and times (10-48 h). The autolyzed material resulted in 3 distinct phases after high speed centrifugation. The intermediate phase (water-phase) was collected and concentrated by membrane filtration. The high-molecular-weight substances (greater than 10,000 Daltons) were further purified by ion-exchange chromatography and eluted by a salt gradient. In this matter a bulk enzymatic material from krill can be efficiently obtained for further purification of different hydrolytic enzymes (peptide hydrolases/carbohydrases).
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