A Simple Protocol for Single Lung Cancer Cell Isolation-Making the Single Cell Based Lung Cancer Research Feasible for Individual Investigator

Abstract

There are no two identical cells in any organism and with human more so. Quantitative measurements of functions in single cell is a critical challenge in any modern biological study. Now with the development of single cell isolation technology, the era of single cell based research is coming, especially when combination with omics science, which takes full advantage of both techniques, and gets rid of their disadvantages. There are several methods for single cell isolation: serial dilution, micromanipulation (mechanical-using a simple mouth pipette and optimal), fluorescence-activated cell sorting (FACS), laser-capture microdissection (LCM) and microfluidic devices. But some techniques need very expensive equipments, which is not easily available for most investigators. Here we developed a simple protocol to isolate and culture single lung cancer cell. This will make single cell-based lung cancer research much more available for individual investigator.