Abstract
For the past several years we have been attempting to study the secondary and tertiary structure of tRNAs + + Abbreviations used in this paper are the same as those used by RajBhandary, Stuart and Chang (1968) with the following additions: BD-cellulose, benzoylated DEAE cellulose; pmole, 10 −12 mole. by means of chemical probes. In the course of this work it became necessary to obtain a highly purified species of tRNA. For two reasons we chose to purify yeast Phenylalanine tRNA. The nucleotide sequence of this molecule has been completely worked out ( RajBhandary and Chang [1968]). Secondly, a simple and highly effective partial purification of this species has been obtained by Gillam et al. (1967) on benzoylated DEAE cellulose. In this communication we report the use of BD-cellulose to complete the purification of tRNA Phe.
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