Abstract
A strain of Pseudomonas aureofaciens (SBW25EeZY-6KX) that was chromosomally marked with a lacZY and a km r -xylE cassette could be recovered from non-sterile soil by a selective Pseudomonas enrichment broth amended with 100 ppm kanamycin and 50 ppm X-gal in an MPN (most probable number) assay. The assay was sensitive and reliable, allowing detection of as few as one recombinant cell in a 1 % (w/v) soil suspension. The soil used contained a large (ca 5% of total culturable bacteria) background of indigenous bacteria that were either able to utilize lactose, were resistant to kanamycin, or both
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