A simple monophasic LGPY medium for routine maintenance of Leishmania donovani promastigotes

Abstract

Here, we report a simple, economic and autoclavable monophasic LGPY medium supplemented with 10% fetal bovine serum (FBS), for routine maintenance of Leishmania donovani promastigotes for laboratory use. In comparison to commercially available M199 and RPMI-1640 media, LGPY has shown approximately sevenfold more cell growth. The parasite has been observed to survive in the medium for at least 15days post-inoculation. The medium also supports long-term sub-passaging of the promastigotes and can also be stored at 4°C or room temperature for 14months and 45days, respectively.