Abstract
An existing enzymic method for preparing [ γ- 32P]ATP from 32 P i has been modified toyield [ γ- 32P]ATP free of salt and buffer. 32P is incorporated into the γ-position of ATP by isotopic exchange in the presence of glyceraldehyde 3-phosphate dehydrogenase and 3-phosphoglycerate kinase. Unreacted 32 P i is separated from [ γ- 32P]ATP by column chromatography on Dowex 1 bicarbonate. [ γ- 32P]ATP is eluted with 2 m triethylammonium bicarbonate, which is then completely removed by freeze-drying.
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