Abstract
A spinning cup sequencer was converted into a vapour-liquid-solid phase instrument by replacing the chemical delivery valves with a simplified version of the Wittmann valve. The cup assembly was replaced by a Kel-F cartridge and the vacuum system slightly modified and fitted with a cold trap. The modification is inexpensive, easily performed and does not lead to the loss of the spinning cup capability of the instrument. The modified sequencer is easy to operate, reduces consumption of chemicals and exhibits a cycle time of 1 h. Small and larger peptides in amounts from 0.2–30 nmol were successfully degraded. Spinning cup protein sequencer Vapour-phase sequencer Protein structure
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