Abstract
As an alternative to the conventional ZAF correction for microprobe analysis, a combined absorption and atomic number correction can be obtained from ø(ρz) curves. This can be easily applied to the analysis of bulk frozen-hydrated biological samples using inorganic standards. It is shown that the method is accurate by analysis of a number of organic and inorganic compounds and gelatine models. Because the corrections vary little with changes in protein concentration over the normal cellular range, iterative ø(ρz) computations are not necessary and the correction procedure is therefore fast and simple.
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