Abstract
Thick blood films cannot be satisfactorily stained by conventional Giemsa methods until they have been dried for several hours. In thick films which are insufficiently dried diluted Giemsa stain produces a marked dispersion of the cytoplasm of leucocytes and of blood protozoa. Prolonged drying greatly reduces this dispersion, hence the usual recommendation that thick blood films should be dried for several hours before staining. Similar preservation of cytoplasmic contour is obtainable by treating freshly dried films for 1 second with an isotonic solution of methylene blue. This procedure obviates delay from slow drying and is perhaps of interest to those who, because of the delay, hesitate to use thick blood films for routine malarial diagnosis.
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