Abstract
The present study aims at building a miniature mass method for the simultaneous determination of 12 phenols including the subtypes of bibenzyl, phenanthrene, and fluorenone, which was used to evaluate the quality of Dendrobium chrysotoxum. Through the full scan mode, new compounds were elucidated. The new compounds were quantified by carrying out the analysis of the ratio of the standard solution areas to new compound areas versus analyte concentration. The limit of detection (LOD) and limit of quantification (LOQ) for phenols were 0.5 µg/mL–1 µg/mL and 1 µg/mL–2 µg/mL, respectively. Average recoveries of phenols were ranged from 83.2% to 97.5%. Reproducibility represented by the RSD percentage was from 2.3% to 8.7%. The average content of the four analytes, erianin, chrysotobibenzyl, confusarin, and moscatilin, were more than 200 mg/kg, and the content of bibenzyl compounds was found to be the highest in Dendrobium chrysotoxum. Among these bibenzyl compounds, erianin was determined as the typical chemical marker from Dendrobium chrysotoxum. The newly established UPLC with a miniature mass detector method was found to be an appropriate tool for the quality assessment of Dendrobium chrysotoxum.
Highlights
Introduction ere are74 species of Dendrobium reported in China [1]; only few Dendrobium species are edible
Erefore, we developed and validated a miniature mass method for the simultaneous determination of 12 phenols including the subtypes of bibenzyl, phenanthrene, and fluorenone, which was used for subsequent analysis of their content in Dendrobium chrysotoxum. rough the full scan mode, new compounds were monitored and quantified based on the ratio of standard solution areas to new compounds. e combination of a fast, low-cost extraction procedure and a rapid detection method gives a reliable screening method that can be applied in the routine laboratory analysis for the determination of these phenols, reducing the cost of analyses and increasing the sample throughput
(1) Full-Scan Spectra of Standards: miniature mass detector (MMD) Conditions. e ESI source conditions were as follows: source temperature was set at 350 °C. e full-scan mode was performed in the range of 50–400 Da, the scan time rate was 8 pin/sec, and the capillary voltage was set to 0.8 kV, while the cone voltage was at 15 V. e positive and negative modes were simultaneously recorded (Table 1)
Summary
Erianin, chrysotobibenzyl, tristin, coumarin, naringenin, apigenin, moscatin, and confusarin were of the highest purity (purity > 98%) were supplied by Zhong Ke Technology Co. Ltd. E stock solutions of the reference compounds were prepared by dissolving the compounds in methanol, and the working standard solutions were prepared daily from stock solutions by diluting the solution with the appropriate volume of the mobile phase. All solutions were stored in a refrigerator at −20°C. HPLC-grade acetonitrile and methanol were provided by Tedia Company Inc. Water was purified using a Milli-Q system (Millipore, Bedford, USA). E other solvents, purchased from Shanghai Chemical Factory (Shanghai, China), were of analytical grade Water was purified using a Milli-Q system (Millipore, Bedford, USA). e other solvents, purchased from Shanghai Chemical Factory (Shanghai, China), were of analytical grade
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