Abstract
A small scale equilibrium dialysis method for the measurement of the nonprotein bound cortisol in plasma is described. Dialysis is carried out in PTFE cells against an isosmolar Hepes buffer, equilibration occurring within 18 hours. Inter-assay variation was found to be approximately 6%. Samples taken from normal males and females and from oestrogen-treated females were analysed and the results compared with published levels. A significant difference in both the total and free cortisol levels was found between the normals and oestrogentreated females.
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