Abstract
The insulin-binding potency of guinea pig anti-insulin serum (GPAIS) is determined following reaction for 30 minutes at room temperature between solutions containing GPAIS and mixtures of unlabeled and131I-labeled insulin. Insulin which has not reacted with the antibodies is extracted from solution by a suspension of finely divided cellulose. From the radioactive contents of supernatant solutions from reaction mixtures containing the sample, an excess of non-precipitating (reference) GPAIS, and the buffered diluent, the potency of the sample can be determined. Factors affecting the assay procedure are considered and it is shown that, within defined limits, results are reproduceable and the method sensitive and rapid. The technique has potential uses for the study of insulin-antibody reactions and has practical applications in the production and rational use of GPAIS.
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