Abstract
We developed a simple and fast LC method quantifying lidocaine and its metabolite monoethylglycinexylidide (MEGX) in plasma. An extraction of liquid–liquid with dichloromethane and sodium hydroxide, was done using 500 μL of plasma or serum, with a UV detection at 210 nm. The results showed linearity of r = 0.996 for lidocaine and 0.998 for MEGX. Precision and accuracy showed coefficient of variation within an acceptable range. Stability test was acceptable for a period of 90 days. Levels were assayed for two patients, in the first, 1,256.14 ng mL−1 of lidocaine and 17.79 ng mL−1 of MEGX were obtained while in the second the levels for the substances were 1,194.33 and 10.47 ng mL−1. This simple and fast method is reliable and can be recommended for the quantification of lidocaine and MEGX in plasma.
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