Abstract
We describe a rapid and simple method for measuring protein degradation in bacteria. After cells have incorporated [1- 14C]leucine, they are washed and resuspended in a medium containing unlabelled leucine. After an incubation period, the cell suspensions are treated with chloramine-T to decarboxylate the [ 14C]leucine released in the course of protein degradation. The 14CO 2 is trapped and counted.
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