Abstract
Abstract We have devised a simple method for introducing macromolecules into the blastocoel of living starfish embryos. In this method, embryos are treated for 15 min with Ca2+ free sea water (CFSW) containing 0.1–5.0 mg/ml of enzymes or immunoglobulins. These molecules are considered to flow through the septate junction, the septa of which are made diffuse by deprivation of Ca2+ ions from the environmental sea water. Introduction of enzymes which affect the component molecules of the extracellular matrix (ECM) drastically deformed the larval morphology. Introduction of a monoclonal antibody recognizing an ECM component prevented the mesenchyme cells from stretching and migrating normally. These results show that our method can be used to study the function of various ECM components in embryonic development.
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