A Simple Method for Gene Expression and Chromatin Profiling of Individual Cell Types within a Tissue

Abstract

(Developmental Cell 18, 1030–1040; June 15, 2010) In Figure 4, the legend incorrectly identified a gene shown in panel E as At5g70450; this locus is in fact At1g70450. The figure legend has been corrected below. Figure 4. Cell Type-Specific Differences in Histone Methylations and Their Relationship to Gene Expression (E and F) Comparison of H3K4me3 and H3K27me3 profiles in H and NH cells on two H cell-enriched genes, At1g70450 and At3g49960, respectively. Genes above the x axis are on the top strand whereas those below the line are on the bottom strand. Dotted boxes surround the gene of interest in each case. A Simple Method for Gene Expression and Chromatin Profiling of Individual Cell Types within a TissueDeal et al.Developmental CellJune 15, 2010In BriefUnderstanding the production and function of specialized cells during development requires the isolation of individual cell types for analysis, but this is currently a major technical challenge. Here we describe a method for cell type-specific RNA and chromatin profiling that circumvents many of the limitations of current methods for cell isolation. We used in vivo biotin labeling of a nuclear envelope protein in individual cell types followed by affinity isolation of labeled nuclei to measure gene expression and chromatin features of the hair and non-hair cell types of the Arabidopsis root epidermis. Full-Text PDF Open Archive