A Simple Method for Extraction of the Horse Feces Virome DNA, Suitable for Oxford Nanopore Sequencing

Abstract

Metagenomics of the viromes associated with symbiotic microbial communities is a rapidly expanding field of research. Most of the relevant studies deal with the viral communities of human or animal feces. The novel technology of long-reads sequencing from Oxford Nanopore that became available during the last years provides outstanding new possibilities for research of uncultured viral communities. However, compared to classical NGS sequencing, this technology is much more sensitive to the quantity and quality of sequenced DNA. We developed a simple, cheap, and efficient protocol for extraction of total virome DNA from horse feces yielding the DNA samples suitable for long-read sequencing. This method can be adapted for the work with other difficult objects.