Abstract
A simple but efficient method for determination of labile protons in drug metabolites using post-column infusion of deuterium oxide (D 2O) in liquid chromatography/mass spectrometry (LC/MS) experiments with electrospray ionization and time-of-flight mass spectrometry is described. The number of exchangeable protons in analytes, i.e. hydroxyl, amine, thiol and carboxylic acid protons, can easily be determined by comparing the increase in m/ z values after H/D-exchange occurring online between a HPLC column and electrospray ion source. Especially, the hydroxyl metabolites and S/N-oxides with the same accurate mass can be distinguished. A good degree of exchange was obtained in repeatable experiments. Only a low consumption of D 2O is needed in a very easy and rapidly set-up procedure. The method is applied in the study of metabolites of omeprazole and imipramine in human and mouse in vitro samples, respectively, together with exact mass data obtained from time-of-flight mass spectrometric experiments.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
