Abstract
A new membrane-filter technique, suitable for field-surveys, was developed to quantify S-reducing bacteria in waters and soil or sediment samples. Samples, diluted if necessary; were passed through S-coated filters, previoulsy coated with a Fe(OH) 2 film. These filters were incubated in screw-cap test-tubes containing a selective medium, in which sodium acetate was the sole electron source. Blackening of the filter, resulting from iron monosulfide production was used as internal growth indicator, and the times necessary for total blackening of each filter to occur were correlated to the initial numbers of viable cells deposited on it.
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