A simple medium for the propagation and maintenance of human diploid cell strains.

Abstract

THE experiences of many workers propagating human diploid cell strains (HDCS) suggest that these cells are uncommonly exacting in their medium requirements1,4. Eagle's basal medium (BME)2 and minimum essential medium (MEM)3 have given most satisfactory growth, but careful adherence to these formulae in preparing the media has not always guaranteed their suitability4. Differences in the purity of the constituents may account for the variability of different batches of media4. The use of standardized Eagle's media4 has largely overcome these difficulties, but it is of interest to report that a simplified Eagle's medium has also been used successfully for the growth and maintenance of cells in our laboratory.