A simple medium for quick growth of Rhizobium japonicum

Abstract

Rhizobium japonicum is a slow-growing Rhizobium in commonly used media. A medium, given in this communication, enables the species to grow quickly, as evidenced by the counts. Various synthetic media are being used lor the growth of Rhizobium spp. (Bergensen 1901, Fred et al. 1932, Iswaran and Jauhri 1969, Iswaran et al. 1969, Norris 1959, West and Wilson 1939, Wilson and Umbreit 1940). In the Netherlands, van Schreven (1958) used calcium glycerophosphate as a constituent of the medium in large-scale manufacture for cultures. Vincent, Jancey and Roughley (per. communication) used costly galactose or arabinose, sterilized by filtration, as a carbon source for slow-growing species. Most of the fast-growing strains of rhizobia can be grown in an excess of 250 × 10 6 viable cells/ml. in 3 days at 28 °C in the usual medium (Fred, Baldwin, and McCoy 1932), while slow-growing types take at least 7 days in this medium to reach the above munber. However, no cheap and efficient medium for quick growth of strains of slow-growing Rhizobium japonicum is available. In the present an attempt has been made investigation in this direction. Rhizobium japonicum ist auf gewöhnlichen Nährmedien als ein langsam wachsendes Knöllchenbakterium zu betrachten. Das in dieser Mitteilung angegebene Medium ermöglicht ein schnelleres Wachstum dieser Rakteriengattung.