Abstract
A simple, relatively inexpensive method for distinguishing Candida albicans from other species of the genus Candida was developed. The organisms were first spread on a solid 2% agar medium which contained 1% bactopeptone, then incubated at 37d`C for three hours and finally examined microscopically. A coverslip placed over the organisms at the time of inoculation increased from 93.5 to 100% the proportion of isolates of C. albicans which formed parallel-sided germ tubes when tested in this way. Five other species of Candida (parapsilosis, krusei, tropicalis. pseudotropicalis and guilliermondii) were also tested but did not form germ tubes. Organisms of C. tropicalis sometimes formed elongated buds but these could be readily distinguished on direct microscopic examination from germ tubes by the constriction at their base.
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