Abstract

Test chambers for conducting in situ fish bioassays were constructed from 81. polyethylene bottles. Yearling fathead minnows (Pimephales promelas) and young-of-the-year bluegill (Lepomis macrochirus) demonstrated greater than 50% survival in the chambers after 65 days of exposure in a reservoir, river and creek. Fathead minnow survival was substantially greater than that of bluegils. The chambers provide a simple, inexpensive, sensitive technique to screen effluents for toxicity.

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