Abstract
Omperazole was used for the treatment of stomach and gastroesophageal reflux disease. Omperazole was used administered in the form of oral dose as independent or combination form. Presnt study was carried out to develop and validate a simple HPLC methtod of determination of omeprazole in vitro. Mobile phase empolyed was acetonitrile : phosphate buffer (65:35), pH 6.8 and C 18 column was used. UV detector was used using 300nm eluted with the mobile phase 1.0 mint/ml. percentage recovery was 99.92%±0.4%. This method was used for the routine and raw material analysis.
Highlights
Omeprazole (5-methoxy-2-[[(4-methoxy-3,5dimethylpyridin-2yl) methyl] sulphinyl]-1Hbenzimidazole) is proton pump inhibitor, deccreases the amount of acid produced in the stomach
Reverse phase HPLC methods were reported for the determination of omeprazole 10
The injection volume was kept at 20μg/mL & pH of the mobile phase was adjusted at 6.8 using phosphate buffer
Summary
Omeprazole (5-methoxy-2-[[(4-methoxy-3,5dimethylpyridin-2yl) methyl] sulphinyl]-1Hbenzimidazole) is proton pump inhibitor, deccreases the amount of acid produced in the stomach. Reverse phase HPLC methods were reported for the determination of omeprazole 10. 2.3 Drug Standard Stock Solution: Internal standard stock solution was prepared by dissolving 100mg of reference standard in the 100 ml of AR grade ethanol. This stock standard solution was further diluted up to the fine concentration of 20μg/ml. 2.7 Linearity: Linearity of newly developed method was observed by analyzing the eight solutions (4, 8, 12, 16, 20, 24, 28, 32, 36 μg/ml).
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