A simple, high-throughput modeling approach reveals insights into the mechanism of gametophytic self-incompatibility.

Jahanshah Ashkani,D J G Rees

doi:10.1038/srep34732

Abstract

Specificity in the GSI response results from the S-haplotype-specific molecular interaction of S-locus F-box (SLF/SFB) and SRNase proteins in the self-incompatibility locus (S-locus). The answer to the question of how these two components of the S-locus (SRNase and SLF/SFB) interact has been gathered from several models. Since there is not enough evidence as to which one is the definitive model, none of them can be ruled out. Despite the identification of interacting protein elements, the mechanism by which SLF/SFB and SRNase interact to differently trigger the self-incompatibility among families and subfamilies remain uncertain. The high-throughput modeling approach demonstrates structural visions into the possible existence of a Collaborative Non-Self Recognition model in apple. These findings postulate several prospects for future investigation providing useful information to guide the implementation of breeding strategies.

Highlights

Obstacle for the breeding of apple by restricting crosses between apple cultivars
As such the knowledge of GSI as to how the SLF/specific F-box (SFB) and SRNase proteins interact, can provide useful information to guide the implementation of breeding strategies. To address this issue a model of interaction between S-locus genes is introduced by assessing the Collaborative Non-Self Recognition model in apple. This model aims at describing the specificities of SLF/SFB and their brothers (SFBBs) in recognizing a large repertoire of SRNases, using molecular modeling and docking techniques
It is concluded that these predicted structures have good homology with the experimentally solved structures of the RNase T2 family enzymes

Summary

Introduction

Obstacle for the breeding of apple by restricting crosses between apple cultivars As such the knowledge of GSI as to how the SLF/SFB and SRNase proteins interact, can provide useful information to guide the implementation of breeding strategies. To address this issue a model of interaction between S-locus genes is introduced by assessing the Collaborative Non-Self Recognition model in apple. This model aims at describing the specificities of SLF/SFB and their brothers (SFBBs) in recognizing a large repertoire of SRNases, using molecular modeling and docking techniques

Methods

Results

Conclusion