Abstract
Problems of applying continuous gradient elution to the adsorption chromatography of lipids and other lipophilic compounds are discussed. A simple and versatile method for producing continous concentration gradients from pairs of eluents of unequal density is described. The method makes use of a nomogram and common pieces of laboratory glassware, such as bottles, measuring cylinders, and chromatographic column tubes. It ahs been found satisfactory for linear, convex, and concave gradients whichever eluent is the denser.
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