A simple detection platform based on molecularly imprinted polymer for AFB1 and FuB1 mycotoxins

Abstract

The sensitive electrochemical sensing platforms were fabricated using molecularly imprinted polymer (MIP) based technique for mycotoxins [Aflatoxin B1 (AFB1) and Fumonisin B1 (FuB1)] detection. Polyaniline was used as a MIP matrix synthesized using the chemical oxidative polymerization method in the presence of AFB1 and FuB1toxins as the template molecules. Fourier transform infrared spectroscopy and electrochemical techniques were used for the characterization of the synthesized MIPs. The proposed biosensors exhibited good sensitivity of 7.037 and 9.092 µA mL ng−1 cm−2 with the lower detection limits of 0.313 and 0.322 pg mL−1 for AFB1 and FuB1. Both sensors showed good linearity amidst the sensing range of 1 pg mL−1 to 500 ng mL−1. The fabricated sensors open up a promising strategy to detect AFB1 and FuB1 as an alternative for biomolecules with enormous specificity, quick responsiveness, and cost-effectiveness. These fabricated sensors manifest good analytical performance compared to other reported MIP-based sensors for AFB1 and FuB1 detection.