Abstract
A method is described of cryopreservation of human lymphocytes for cytogenetic studies. An aliquot of whole blood is pre-incubated in a PHA-added medium for at least one hour at 37°C, and then stored frozen at −140°C in Eagle's MEM containing 10% fetal bovine serum and 10% DMSO for various periods up to one year. Thawing is made quickly in a water-bath at 37°C. After thorough washing with MEM, the routine PHA-added blood culture method is applied, and airdried chromosome preparations are made. The results are highly reproducible with a good number of mitotic cells sufficient for karyotyping.
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Schedule a callMore From: Jinrui idengaku zasshi. The Japanese journal of human genetics
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