Abstract
A method for cryopreserving a 100-μm-thick sheet of tissue produced by cultured rabbit chondrocytes has been developed. The method maintains cell viability and avoids tissue fracture and degradation of mechanical properties. A slow-freeze, fast-thaw procedure with 2 M Me2SO as the cryoprotectant resulted in no tissue fracture and approximately 90% viable cells after storage in culture flasks at −80°C. The cells in the retrieved tissue remained responsive to IL-1β, and tensile and fracture toughness properties of the tissue were not degraded by cryopreservation.
Full Text 
Sign-in/Register to access full text options
Sign-in/Register to access full text options 
Published version (
Free)
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
