A simple, cost-effective colorimetric assay for aluminum ions via complexation with the flavonoid rutin

Abstract

Aluminum has been linked to deleterious health effects with high concentration, chronic exposure, creating a need for innovative detection techniques. Colorimetric assays are an ideal approach since they are simple, cost-effective, and field adaptable. Yet, commercially available colorimetric assays for aluminum are limited since it forms few colored chelation complexes. Flavonoids, a class of polyphenolic compounds, are one of the few examples that create colored aluminum complexes. Aluminum ions (Al3+) are the main constituent in colorimetric assays for flavonoid detection in food or plant samples. Our assay design was based on colorimetric flavonoid assays, where the assay reported herein was optimized. Specifically, the flavonoid rutin concentration and sample-to-rutin volume ratio (295:5 µL) were optimized to detect Al3+ at low µM concentrations in samples. The assay performed comparably, and in some instances better, than those requiring advanced instrumentation and previously reported colorimetric assays, with a linear range (1–8 µM), sensitivity (7.6 nM), limit of detection (79.8 nM), and limit of quantification (266 nM) for Al3+. The colorimetric assay was accurate (99 ≤ 108 ± 4 ≤ 6% Al3+ recovery), precise (low intra- and inter-assay coefficient of variation (CV) of 3.1 ≤ 5.9% and 4.4%, respectively), and selective for Al3+ ions compared to solutions containing a variety of other mono-, di-, and tri-cations at much higher concentrations (10- to 100-fold higher). Lastly, the colorimetric assay was applicable to complex analysis. It was used to generate a chelation curve depicting the Al3+ chelation capacity of sodium alginate, a biologically derived polymer used as a bioink for 3D bioprinting.