Abstract
A new method is described for the combined determination of β-1,3-glucan and cell wall polysaccharides in diatoms, representing total cellular carbohydrate. The glucan is extracted by 0.05 mol l−1 H2SO4 at 60 °C for 10 min, and the cell wall polysaccharides are subsequently hydrolyzed by 80% H2SO4 at 0–4 °C for 20 h. Each carbohydrate fraction is determined by the phenol-sulphuric acid method. The method has been demonstrated for axenic cultures of the marine diatom Skeletonema costatum and natural marine phytoplankton populations dominated by diatoms. Cellular glucan and cell wall polysaccharides were determined with standard deviations of 1–3% and 2–5%, respectively.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
