Abstract
A simple sensitive and rapid colorimetric method has been developed, and herein described, for the qualitative and quantitative chemical assessment of the commercially available chitosan products. The described method relies on the reactivity of the basic amino function of chitosan with the acid dye bromocresol purple. The applied technique allows assessment of variability and selectivity changes in the quality of the marketed chitosan products.
Highlights
Chitosan is a natural biocompatible polymer derived from the naturally-occurring polysaccharide-based biopolymer, chitin, by deacetylation with an alkali leaving behind a free amino group (-NH2) (Figure 1) [1]
Results tercept and slope values of DEAE-cellulose in comparison to those obtained with chitosan products tip off the analyst to the performance properties, efficiency and capacity of such polymers as anionic exchangers relative to a different match of known operational parameters
The acetylated chitosan matrix have acquired a red color once wetted with bromocresol purple, yet, the acidic solution used to drive out the adsorbed dye did not release any noticeable color intensity
Summary
Chitosan is a natural biocompatible polymer derived from the naturally-occurring polysaccharide-based biopolymer, chitin, by deacetylation with an alkali leaving behind a free amino group (-NH2) (Figure 1) [1]. Chitosan naturally exists only in a few species of fungi but it is mainly extracted from the cuticular and exoskeletons of invertebrates like crustaceans, mollusks, crabs and shrimp. The biopolymer chitosan is composed of β-2-amino-2-deoxy-D-glucopyranose(glucosamine units) and β-2-acetamido-2-deoxy-D-glucopyranose [2]. Chitosan possesses unique properties like its ability to form films, and possesses a positive ionic charge which develops its ability to chemically bind with negatively charged fats, lipids and bile acids. The degree of deacetylation is one of the most important chemical characteristics as it reported to influence the physicochemical properties and the performance of chitosan in many of its applications. Chitosan versatility depends mainly on the chemically reactive amino groups
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