Abstract
Various clinical and laboratory experiments have demonstrated that hepatoma specific gamma-glutamyltransferase isoenzyme (HS-GGT) is a specific and sensitive biomarker in monitoring the liver carcinogenesis. Various methods for detection of HS-GGT, such as stage gel electrophoresis or chromatography, have been set up; however, these methods are complicated. To set up a method convenient for detection of HS-GGT isoenzyme, we established a method of datura stramonium agglutinin (DSA)-sepharose affinity chromatography to detect HS-GGT in serum, and further investigate its diagnostic value in 154 patients with primary hepatocellular carcinoma and benign liver diseases and 21 healthy subjects. Results showed this method could distinguish hepatocellular carcinoma from extrahepatic tumors and benign liver diseases such as liver cirrhosis and hepatitis B. Using DSA-sepharose affinity chromatography is a simple method with similar diagnostic sensitivity and specificity compared with other methods for detection of HS-GGT.
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