Abstract
A simple, precise, rapid, selective, and economic high-performance thin-layer chromatographic (HPTLC) method has been established for simultaneous analysis of domperidone (DMP) and paracetamol (PAR) in tablet dosage forms. The chromatographic separation was performed on precoated silica gel 60 GF 254 plates with acetone-toluene-methanol 4:4:2 ( v/v ) as mobile phase. The plates were developed to a distance of 8.0 cm at ambient temperature. The developed plates were scanned and quantified at their single wavelength of maximum absorption at approximately 285 and 248 nm for domperidone and paracetamol, respectively. Experimental conditions such as band size, chamber saturation time, migration of solvent front, slit width, etc. were critically studied and the optimum conditions were selected. The drugs were satisfactorily resolved with R F 0.52 ± 0.02 for domperidone and 0.74 ± 0.02 for paracetamol. The method was validated for linearity, accuracy, precision, and specificity. The calibration plot was linear b...
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