Abstract
Multiplex PCR methods have been frequently used for authentication of meat product adulteration. Through screening of new species-specific primers designed based on the mitochondrial DNA sequences, a septuple PCR method is ultimately developed and optimized to simultaneously detect seven species including turkey (110 bp), goose (194 bp), pig (254 bp), sheep (329 bp), beef (473 bp), chicken (612 bp) and duck (718 bp) in one reaction. The proposed method has been validated to be specific, sensitive, robust and inexpensive. Taken together, the developed septuple PCR assay is reliable and efficient, not only to authenticate animal species in commercial meat products, but also easily feasible in a general laboratory without special infrastructures.
Highlights
Meat authentication is an important concern to protect consumers from illegal and unwanted ingredients [1,2,3,4]
Each set of primers was compared against 16 species by simplex polymerase chain reaction (PCR) assays
To further test the efficiency and specificity of primers, simplex PCRs were carried out using a DNA mixture of all seven meat species
Summary
Meat authentication is an important concern to protect consumers from illegal and unwanted ingredients [1,2,3,4] Meat adulteration such as unlisted, mislabeled or fraudulent ingredients has frequently been reported around the world and has become a severe global issue [4,5]. Meat adulteration could violate religious concerns; as is known, meat products containing pork ingredients are not permitted by Kosher and Halal food laws [10,11]. It remains a pressing need for identifying meat species with 100% accuracy in real-world foodstuffs
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